GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:15686447
| Citation |
Sydorskyy, Y, Dilworth, DJ, Halloran, B, Yi, EC, Makhnevych, T, Wozniak, RW and Aitchison, JD (2005) Nop53p is a novel nucleolar 60S ribosomal subunit biogenesis protein. Biochem. J. 388:819-26 |
|---|---|
| Abstract |
Ribosome biogenesis in Saccharomyces cerevisiae occurs primarily in a specialized nuclear compartment termed the nucleolus within which the rRNA genes are transcribed by RNA polymerase I into a large 35 S rRNA precursor. The ensuing association/dissociation and catalytic activity of numerous trans-acting protein factors, RNAs and ribosomal proteins ultimately leads to the maturation of the precursor rRNAs into 25, 5.8 and 18 S rRNAs and the formation of mature cytoplasmic 40 and 60 S ribosomal subunits. Although many components involved in ribosome biogenesis have been identified, our understanding of this essential cellular process remains limited. In the present study we demonstrate a crucial role for the previously uncharacterized nucleolar protein Nop53p (Ypl146p) in ribosome biogenesis. Specifically, Nop53p appears to be most important for biogenesis of the 60 S subunit. It physically interacts with rRNA processing factors, notably Cbf5p and Nop2p, and co-fractionates specifically with pre-60 S particles on sucrose gradients. Deletion or mutations within NOP53 cause significant growth defects and display significant 60 S subunit deficiencies, an imbalance in the 40 S:60 S ratio, as revealed by polysome profiling, and defects in progression beyond the 27 S stage of 25 S rRNA maturation during 60 S biogenesis. |
| Links |
PubMed PMC1183461 Online version:10.1042/BJ20041297 |
| Keywords |
Cell Nucleolus/chemistry; Cell Nucleolus/metabolism; Gene Expression Regulation, Fungal; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Polyribosomes/genetics; Polyribosomes/metabolism; Protein Subunits/chemistry; Protein Subunits/metabolism; RNA, Fungal/genetics; RNA, Fungal/metabolism; RNA, Ribosomal/genetics; RNA, Ribosomal/metabolism; Ribosomes/chemistry; Ribosomes/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
