PMID:15654330

Citation	Belyantseva, IA, Boger, ET, Naz, S, Frolenkov, GI, Sellers, JR, Ahmed, ZM, Griffith, AJ and Friedman, TB (2005) Myosin-XVa is required for tip localization of whirlin and differential elongation of hair-cell stereocilia. Nat. Cell Biol. 7:148-56
Abstract	Stereocilia are microvilli-derived mechanosensory organelles that are arranged in rows of graded heights on the apical surface of inner-ear hair cells. The 'staircase'-like architecture of stereocilia bundles is necessary to detect sound and head movement, and is achieved through differential elongation of the actin core of each stereocilium to a predetermined length. Abnormally short stereocilia bundles that have a diminished staircase are characteristic of the shaker 2 (Myo15a(sh2)) and whirler (Whrn(wi)) strains of deaf mice. We show that myosin-XVa is a motor protein that, in vivo, interacts with the third PDZ domain of whirlin through its carboxy-terminal PDZ-ligand. Myosin-XVa then delivers whirlin to the tips of stereocilia. Moreover, if green fluorescent protein (GFP)-Myo15a is transfected into hair cells of Myo15a(sh2) mice, the wild-type pattern of hair bundles is restored by recruitment of endogenous whirlin to the tips of stereocilia. The interaction of myosin-XVa and whirlin is therefore a key event in hair-bundle morphogenesis.
Links	PubMed Online version:10.1038/ncb1219
Keywords	Actins/metabolism; Animals; COS Cells; Cells, Cultured; Cercopithecus aethiops; Cilia/ultrastructure; Deafness/genetics; Green Fluorescent Proteins; Hair Cells, Auditory, Inner/growth & development; Hair Cells, Auditory, Inner/ultrastructure; Membrane Proteins/metabolism; Mice; Mice, Mutant Strains; Models, Biological; Molecular Motor Proteins/metabolism; Myosins/physiology
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.