PMID:15581879

Citation	Parlato, R, Rosica, A, Rodriguez-Mallon, A, Affuso, A, Postiglione, MP, Arra, C, Mansouri, A, Kimura, S, Di Lauro, R and De Felice, M (2004) An integrated regulatory network controlling survival and migration in thyroid organogenesis. Dev. Biol. 276:464-75
Abstract	The thyroid gland originates from the ventral floor of the foregut as a thickening of the endodermal cell layer. The molecular mechanisms underlying the early steps of thyroid morphogenesis are not known. Gene targeting experiments have contributed to the identification of several transcription factors, in general playing a role in the proliferation, survival, and migration of the thyroid cell precursors. The experiments reported here analyze the expression of the transcription factors Titf1, Hhex, Pax8, and Foxe1 in the thyroid primordium of null mutants of each of them. We found that most of these transcription factors are linked in an integrated regulatory network, each of them controlling the presence of other members of the network. The expression of Foxe1 is regulated in an intriguing fashion as it is strongly dependent on the presence of Pax8 in thyroid precursor cells, while the expression of the same gene in the pharyngeal endoderm surrounding the primordium is dependent on Sonic hedgehog (Shh)-derived signaling. Moreover, by the generation of mouse mutants expressing Foxe1 exclusively in the thyroid primordium, we provide a better understanding of the role of Foxe1 in these cells in order to acquire the competence to migrate into the underlying mesenchyme. In conclusion, we provide the first evidence of gene expression programs, controlled by a hierarchy of transcription factors expressed in the thyroid presumptive gut domain and directing the progression of thyroid morphogenesis.
Links	PubMed Online version:10.1016/j.ydbio.2004.08.048
Keywords	Animals; Cell Movement; Cell Survival; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Endoderm/cytology; Endoderm/metabolism; Forkhead Transcription Factors; Gene Expression Regulation, Developmental; Hedgehog Proteins; In Situ Hybridization; Mice; Mice, Inbred Strains; Mice, Knockout; Morphogenesis; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Paired Box Transcription Factors; Signal Transduction/physiology; Stem Cells/cytology; Stem Cells/metabolism; Thyroid Gland/cytology; Thyroid Gland/embryology; Thyroid Gland/metabolism; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism
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