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PMID:15574935
| Citation |
Amarita, F, Yvon, M, Nardi, M, Chambellon, E, Delettre, J and Bonnarme, P (2004) Identification and functional analysis of the gene encoding methionine-gamma-lyase in Brevibacterium linens. Appl. Environ. Microbiol. 70:7348-54 |
|---|---|
| Abstract |
The enzymatic degradation of L-methionine and subsequent formation of volatile sulfur compounds (VSCs) is believed to be essential for flavor development in cheese. L-methionine-gamma-lyase (MGL) can convert L-methionine to methanethiol (MTL), alpha-ketobutyrate, and ammonia. The mgl gene encoding MGL was cloned from the type strain Brevibacterium linens ATCC 9175 known to produce copious amounts of MTL and related VSCs. The disruption of the mgl gene, achieved in strain ATCC 9175, resulted in a 62% decrease in thiol-producing activity and a 97% decrease in total VSC production in the knockout strain. Our work shows that L-methionine degradation via gamma-elimination is a key step in the formation of VSCs in B. linens. |
| Links |
PubMed PMC535188 Online version:10.1128/AEM.70.12.7348-7354.2004 |
| Keywords |
Amino Acid Sequence; Brevibacterium/genetics; Brevibacterium/growth & development; Brevibacterium/metabolism; Carbon-Sulfur Lyases/chemistry; Carbon-Sulfur Lyases/genetics; Carbon-Sulfur Lyases/metabolism; Cheese/microbiology; Gene Deletion; Methionine/metabolism; Molecular Sequence Data; Sequence Analysis, DNA; Sulfhydryl Compounds/metabolism; Sulfur Compounds/metabolism; Volatilization |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0018826: methionine gamma-lyase activity |
ECO:0000315: |
F |
Figure 1 shows the l-methionine-gamma-lyase knockout mutant fails to produce methanthiol, alpha-ketobutyrate, and ammonia. Figure 1 also shows the wild type strain produces large amounts of methanthiol, alpha-ketobutyrate. |
complete | ||||
|
enables |
GO:0018826: methionine gamma-lyase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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