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PMID:15541767
| Citation |
Petit, FG, Salas, R, Tsai, MJ and Tsai, SY The regulation of COUP-TFII gene expression by Ets-1 is enhanced by the steroid receptor co-activators. Mech. Ageing Dev. 125:719-32 |
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| Abstract |
Recent phenotypic analysis of orphan nuclear receptor chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) [NR2F2] knockout mice shows that COUP-TFII is involved in the angiogenic process in the developing embryos. Since Ets-1 expression is also correlated with angiogenesis, and both Ets-1 and COUP-TFII mRNA are present in mesenchymal cells, we have sought to determine whether Ets-1 is a potential regulator of COUP-TFII gene expression. For this purpose, we performed transient transfection experiments using a luciferase reporter construct containing the mouse COUP-TFII promoter. We found that the COUP-TFII promoter activity is indeed regulated by Ets-1. We have identified two identical inverted potential ETS-binding sites located 47 nucleotides downstream of the start site. Mutation of both sites reduced the ability of Ets-1 to enhance the COUP-TFII promoter activity. Furthermore, other members of the ETS family such as Ets-2 or ETV1 are also potent regulators of the COUP-TFII promoter. Finally, the induction of the COUP-TFII gene is strongly enhanced by the expression of steroid receptor co-activator factors through a direct interaction with Ets-1. These results indicate that COUP-TFII is a potential downstream target of Ets-1 and it may partially mediate the Ets-1 function in angiogenesis. |
| Links |
PubMed Online version:10.1016/j.mad.2004.03.009 |
| Keywords |
Animals; COUP Transcription Factor II; COUP Transcription Factors; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/genetics; Gene Expression Regulation/genetics; Gene Expression Regulation/physiology; HeLa Cells; Humans; Mice; Neovascularization, Physiologic/genetics; Neovascularization, Physiologic/physiology; Proto-Oncogene Protein c-ets-1; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/metabolism; Proto-Oncogene Proteins c-ets; Receptors, Steroid/biosynthesis; Receptors, Steroid/genetics; Receptors, Steroid/metabolism; Transcription Factors/biosynthesis; Transcription Factors/genetics; Transcription Factors/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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