PMID:15520468

Citation	Bouchoux, C, Hautbergue, G, Grenetier, S, Carles, C, Riva, M and Goguel, V (2004) CTD kinase I is involved in RNA polymerase I transcription. Nucleic Acids Res. 32:5851-60
Abstract	RNA polymerase II carboxy terminal domain (CTD) kinases are key elements in the control of mRNA synthesis. Yeast CTD kinase I (CTDK-I), is a non-essential complex involved in the regulation of mRNA synthesis at the level of transcription elongation, pre-mRNA 3' formation and nuclear export. Here, we report that CTDK-I is also involved in ribosomal RNA synthesis. We show that CTDK-I is localized in part in the nucleolus. In its absence, nucleolar structure and RNA polymerase I transcription are affected. In vitro experiments show an impairment of the Pol I transcription machinery. Remarkably, RNA polymerase I co-precipitates from cellular extracts with Ctk1, the kinase subunit of the CTDK-I complex. In vitro analysis further demonstrates a direct interaction between RNA polymerase I and Ctk1. The results suggest that CTDK-I might participate in the regulation of distinct nuclear transcriptional machineries, thus playing a role in the adaptation of the global transcriptional response to growth signalling.
Links	PubMed PMC528809 Online version:10.1093/nar/gkh927
Keywords	Cell Nucleolus/chemistry; Cell Nucleolus/ultrastructure; Multienzyme Complexes/analysis; Mutation; Protein Kinases/analysis; Protein Kinases/genetics; Protein Kinases/physiology; RNA Polymerase I/metabolism; Transcription, Genetic
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