PMID:15514068

Citation	Johnson, MA, von Besser, K, Zhou, Q, Smith, E, Aux, G, Patton, D, Levin, JZ and Preuss, D (2004) Arabidopsis hapless mutations define essential gametophytic functions. Genetics 168:971-82
Abstract	In flowering plants, the egg develops within a haploid embryo sac (female gametophyte) that is encased within the pistil. The haploid pollen grain (male gametophyte) extends a pollen tube that carries two sperm cells within its cytoplasm to the embryo sac. This feat requires rapid, precisely guided, and highly polarized growth through, between, and on the surface of the cells of the stigma, style, and ovary. Pollen tube migration depends on a series of long-range signals from diploid female cells as well as a short-range attractant emitted by the embryo sac that guides the final stage of tube growth. We developed a genetic screen in Arabidopsis thaliana that tags mutant pollen with a cell-autonomous marker carried on an insertion element. We found 32 haploid-disrupting (hapless) mutations that define genes required for pollen grain development, pollen tube growth in the stigma and style, or pollen tube growth and guidance in the ovary. We also identified genomic DNA flanking the insertion element for eleven hap mutants and showed that hap1 disrupts AtMago, a gene whose ortholog is important for Drosophila cell polarity.
Links	PubMed PMC1448849 Online version:10.1534/genetics.104.029447
Keywords	Arabidopsis/embryology; Arabidopsis/genetics; Arabidopsis Proteins/genetics; Cell Polarity; Flowers/physiology; Genes, Plant; Haploidy; Mutation/genetics; Phenotype; Pollen/physiology
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.