PMID:15492211

Citation	Park, JW, Parisky, K, Celotto, AM, Reenan, RA and Graveley, BR (2004) Identification of alternative splicing regulators by RNA interference in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 101:15974-9
Abstract	Alternative splicing is thought to be regulated by nonspliceosomal RNA binding proteins that modulate the association of core components of the spliceosome with the pre-mRNA. Although the majority of metazoan genes encode pre-mRNAs that are alternatively spliced, remarkably few splicing regulators are currently known. Here, we used RNA interference to examine the role of >70% of the Drosophila RNA-binding proteins in regulating alternative splicing. We identified 47 proteins as splicing regulators, 26 of which have not previously been implicated in alternative splicing. Many of the regulators we identified are nonspliceosomal RNA-binding proteins. However, our screen unexpectedly revealed that altering the concentration of certain core components of the spliceosome specifically modulates alternative splicing. These results significantly expand the number of known splicing regulators and reveal an extraordinary richness in the mechanisms that regulate alternative splicing.
Links	PubMed PMC528766 Online version:10.1073/pnas.0407004101
Keywords	Alternative Splicing; Animals; Cell Adhesion Molecules; Cell Nucleus/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Genes, Insect; Mutation; RNA Interference; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism
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Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
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