PMID:15378064

Citation	Chase, DL, Pepper, JS and Koelle, MR (2004) Mechanism of extrasynaptic dopamine signaling in Caenorhabditis elegans. Nat. Neurosci. 7:1096-103
Abstract	D1-like and D2-like dopamine receptors have synergistic and antagonistic effects on behavior. To understand the mechanisms underlying these effects, we studied dopamine signaling genetically in Caenorhabditis elegans. Knocking out a D2-like receptor, DOP-3, caused locomotion defects similar to those observed in animals lacking dopamine. Knocking out a D1-like receptor, DOP-1, reversed the defects of the DOP-3 knockout. DOP-3 and DOP-1 have their antagonistic effects on locomotion by acting in the same motor neurons, which coexpress the receptors and which are not postsynaptic to dopaminergic neurons. In a screen for mutants unable to respond to dopamine, we identified four genes that encode components of the antagonistic Galpha(o) and Galpha(q) signaling pathways, including Galpha(o) itself and two subunits of the regulator of G protein signaling (RGS) complex that inhibits Galpha(q). Our results indicate that extrasynaptic dopamine regulates C. elegans locomotion through D1- and D2-like receptors that activate the antagonistic Galpha(q) and Galpha(o) signaling pathways, respectively.
Links	PubMed Online version:10.1038/nn1316
Keywords	Acetylcholine/metabolism; Animals; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/isolation & purification; Caenorhabditis elegans Proteins/metabolism; DNA, Complementary/analysis; DNA, Complementary/genetics; Dopamine/metabolism; GTP-Binding Protein alpha Subunits, Gi-Go/genetics; GTP-Binding Protein alpha Subunits, Gi-Go/metabolism; GTP-Binding Protein alpha Subunits, Gq-G11/genetics; GTP-Binding Protein alpha Subunits, Gq-G11/metabolism; GTP-Binding Proteins/genetics; GTP-Binding Proteins/isolation & purification; GTP-Binding Proteins/metabolism; Gene Targeting; Molecular Sequence Data; Motor Activity/genetics; Motor Neurons/metabolism; Mutation/genetics; Nervous System/metabolism; Phylogeny; RGS Proteins/genetics; RGS Proteins/metabolism; Receptors, Dopamine/genetics; Receptors, Dopamine/isolation & purification; Receptors, Dopamine/metabolism; Receptors, Dopamine D1/genetics; Receptors, Dopamine D1/metabolism; Receptors, Dopamine D2/genetics; Receptors, Dopamine D2/isolation & purification; Receptors, Dopamine D2/metabolism; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Signal Transduction/genetics; Signal Transduction/physiology
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.