GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:1531632
| Citation |
Girard, JP, Lehtonen, H, Caizergues-Ferrer, M, Amalric, F, Tollervey, D and Lapeyre, B (1992) GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast. EMBO J. 11:673-82 |
|---|---|
| Abstract |
Among the few proteins of the eukaryotic nucleolus that have been characterized, four proteins, nucleolin, fibrillarin, SSB1 and NSR1, possess a common structural motif, the GAR domain, which is rich in glycine and arginine residues. In order to examine whether the presence of this domain is characteristic of a family of nucleolar proteins, we investigated whether other yeast genes encode proteins containing GAR domains. We report here the sequence and the characterization of a new yeast gene, GAR1, which encodes a protein of 205 residues containing two GAR domains. GAR1 is a non-ribosomal protein, localized in the yeast nucleolus, which is essential for cell growth. Immunoprecipitation with anti-GAR1 antibodies shows that GAR1 is associated with a subset of snoRNAs, including snR10 and snR30. Depletion of GAR1 by expression under the control of a regulated GAL promoter, impairs processing of the 35S primary transcript of pre-rRNA and prevents synthesis of 18S rRNA. GAR1 is thus the fifth member of a family of nucleolar proteins containing GAR domains, and is involved in rRNA metabolism. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Blotting, Southern; Cloning, Molecular; DNA, Fungal/genetics; DNA, Fungal/isolation & purification; Escherichia coli/genetics; Fungal Proteins/genetics; Genes, Fungal; Molecular Sequence Data; Multigene Family; Nuclear Proteins/genetics; RNA Precursors/genetics; RNA Processing, Post-Transcriptional; Recombinant Fusion Proteins/metabolism; Restriction Mapping; Ribonucleoproteins/genetics; Ribonucleoproteins/metabolism; Ribonucleoproteins, Small Nuclear; Ribonucleoproteins, Small Nucleolar; Ribosomes/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; beta-Galactosidase/genetics; beta-Galactosidase/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
