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PMID:15276155

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Citation

Blank, M, Triana-Baltzer, GB, Richards, CS and Berg, DK (2004) Alpha-protocadherins are presynaptic and axonal in nicotinic pathways. Mol. Cell. Neurosci. 26:530-43

Abstract

The protocadherin families pcdh-alpha, beta, and gamma have been proposed to mediate synaptic specificity via homophilic interactions. Here we report isolation of two pcdh-alpha family members from chick. We find pcdh-alpha mRNA in multiple regions of chick CNS including cerebellum, tectum, olfactory bulb, and forebrain, and in the autonomic nervous system. Immunoblots identify major components of 120 and 140 kDa both in brain and ciliary ganglion extracts. Immunohistochemistry reveals pcdh-alphas in axons and perisynaptically in preganglionic terminals, adjacent to transmitter release sites. Pcdh-alphas appear to be absent from postsynaptic sites: They are nonoverlapping with postsynaptic receptor clusters in the ganglion and are rapidly lost after ganglionic denervation. Similar pcdh-alpha patterns are found in motor axons and at neuromuscular junctions of birds and mammals, and persist into adulthood. The results indicate that pcdh-alphas are widely expressed in nicotinic cholinergic pathways and may engage in heterophilic interactions at synapses and on axons.

Links

PubMed Online version:10.1016/j.mcn.2004.04.008

Keywords

Animals; Axons/metabolism; Axons/ultrastructure; Brain/cytology; Brain/embryology; Brain/metabolism; Cadherins/genetics; Cadherins/isolation & purification; Cadherins/metabolism; Cell Line; Chick Embryo; Cholinergic Fibers/metabolism; Cholinergic Fibers/ultrastructure; DNA, Complementary/analysis; DNA, Complementary/genetics; Ganglia, Parasympathetic/cytology; Ganglia, Parasympathetic/embryology; Ganglia, Parasympathetic/metabolism; Humans; Molecular Sequence Data; Molecular Weight; Neural Pathways/cytology; Neural Pathways/embryology; Neural Pathways/metabolism; Presynaptic Terminals/metabolism; Presynaptic Terminals/ultrastructure; Protein Isoforms/genetics; Protein Isoforms/isolation & purification; Protein Isoforms/metabolism; RNA, Messenger/metabolism; Rats; Receptors, Nicotinic/metabolism; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Synaptic Transmission/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:PCD15

part_of

GO:0098981: cholinergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0000010)

Seeded From UniProt

complete

CHICK:Q0ZM04

part_of

GO:0098793: presynapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q0ZM04

part_of

GO:0098981: cholinergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

part_of:(UBERON:0000010)

Seeded From UniProt

complete

CHICK:Q0ZM05

part_of

GO:0098981: cholinergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

part_of:(UBERON:0000010)

Seeded From UniProt

complete

CHICK:Q0ZM05

part_of

GO:0098793: presynapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q0ZM06

part_of

GO:0098981: cholinergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

part_of:(UBERON:0000010)

Seeded From UniProt

complete

CHICK:Q0ZM06

part_of

GO:0098793: presynapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q0ZM07

part_of

GO:0098981: cholinergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

part_of:(UBERON:0000010)

Seeded From UniProt

complete

CHICK:Q0ZM07

part_of

GO:0098793: presynapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q0ZM08

part_of

GO:0098793: presynapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q0ZM08

part_of

GO:0098981: cholinergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

part_of:(UBERON:0000010)

Seeded From UniProt

complete

CHICK:Q0ZM09

part_of

GO:0098793: presynapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q0ZM09

part_of

GO:0098981: cholinergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

part_of:(UBERON:0000010)

Seeded From UniProt

complete

CHICK:Q0ZM10

part_of

GO:0098793: presynapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q0ZM10

part_of

GO:0098981: cholinergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

part_of:(UBERON:0000010)

Seeded From UniProt

complete

CHICK:PCD15

part_of

GO:0098793: presynapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:PCD15

part_of

GO:0098981: cholinergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

part_of:(UBERON:0000010)

Seeded From UniProt

complete

CHICK:Q0ZM12

part_of

GO:0098793: presynapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q0ZM12

part_of

GO:0098981: cholinergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

part_of:(UBERON:0000010)

Seeded From UniProt

complete

CHICK:Q0ZM13

part_of

GO:0098793: presynapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q0ZM13

part_of

GO:0098981: cholinergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

part_of:(UBERON:0000010)

Seeded From UniProt

complete

CHICK:Q0ZM14

part_of

GO:0098793: presynapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q0ZM14

part_of

GO:0098981: cholinergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

part_of:(UBERON:0000010)

Seeded From UniProt

complete

Notes

See also

References

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