PMID:15259052

Citation	Asano, Y, Mizuno, T, Kon, T, Nagasaki, A, Sutoh, K and Uyeda, TQ (2004) Keratocyte-like locomotion in amiB-null Dictyostelium cells. Cell Motil. Cytoskeleton 59:17-27
Abstract	Starved Dictyostelium amoebae continuously change their shape and they are elongated along the front-rear axis during locomotion. In contrast, we found that disruption of the amiB gene, which had been identified as a gene required for the aggregation process during development, caused these cells to move in a manner similar to fish keratocytes. Starved amiB- cells were elongated laterally and had one large lamellipodium along the front side arc of the cell. These cells moved unidirectionally for long distances maintaining the half-moon shape, and this movement followed the predictions of the graded radial extension model, which was originally developed to describe the keratocyte movements. Furthermore, the distributions of actin, Arp2, and myosin II in amiB- cells were similar to those in keratocytes. Therefore, locomotion by keratocytes and amiB- cells appears to be driven by similar mechanisms of cytoskeletal regulation. Double knockout cells lacking both AmiB and myosin II were still able to move unidirectionally in a keratocyte-like manner, although the frequency of those movements was lower. Thus, myosin II is dispensable for the unidirectional movement, though it likely functions in the maintenance of the characteristic half-moon shape. This mutant cell can be a useful tool for further molecular genetic analysis of the mechanism of cell locomotion.
Links	PubMed Online version:10.1002/cm.20015
Keywords	Animals; Cytoskeletal Proteins/genetics; Dictyostelium/genetics; Dictyostelium/physiology; Microscopy, Confocal; Microscopy, Fluorescence; Protozoan Proteins/genetics; Protozoan Proteins/physiology
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.