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PMID:15225124

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Citation

Spooner, RA, Watson, PD, Marsden, CJ, Smith, DC, Moore, KA, Cook, JP, Lord, JM and Roberts, LM (2004) Protein disulphide-isomerase reduces ricin to its A and B chains in the endoplasmic reticulum. Biochem. J. 383:285-93

Abstract

Cells expressing ricin B chain within the secretory pathway are significantly more resistant to intoxication by ricin holotoxin but not to other cytotoxins that exploit similar endocytic routes to the cytosol. Furthermore, cells expressing the related B chain of abrin are protected against both incoming abrin and ricin. These phenotypes can be correlated with the abilities of the respective B chains to form disulphide-linked A-B holotoxins, since abrin B chain forms heterodimers with either abrin or ricin A chains, whereas ricin B chain forms heterodimers with ricin A chain only. In the ricin B-expressing cells, this newly made lectin disappears with biphasic kinetics comprising a retention phase followed by slow turnover and disposal after disengagement from calnexin cycle components. Interference with ricin cytotoxicity occurs during the early retention phase when ricin B chain is associated with PDI (protein disulphide-isomerase). The data show that retrotranslocation of incoming toxin is impeded by PDI-catalysed formation of heterodimers between endogenous B and A chains derived from reduced holotoxin, thus proving that reduction of ricin occurs in the endoplasmic reticulum. In contrast with other toxins, ricin does not appear to require either proteolytic cleavage or unfolding for PDI-catalysed reduction.

Links

PubMed PMC1134069 Online version:10.1042/BJ20040742

Keywords

Abrin/pharmacology; Biological Transport; Dimerization; Disulfides/metabolism; Endocytosis; Endoplasmic Reticulum/metabolism; HeLa Cells; Humans; Oxidation-Reduction; Peptide Hydrolases/metabolism; Proteasome Endopeptidase Complex/metabolism; Protein Disulfide-Isomerases/metabolism; Protein Folding; Protein Structure, Quaternary; Ricin/chemistry; Ricin/genetics; Ricin/metabolism; Ricin/pharmacology; Sulfhydryl Compounds/chemistry; Sulfhydryl Compounds/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:PDIA1

GO:0003756: protein disulfide isomerase activity

ECO:0000314:

F

Figure 8 shows the Oxydoreductase activity of PDI using ricin.

complete
CACAO 4181

HUMAN:PDIA1

enables

GO:0003756: protein disulfide isomerase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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