PMID:15167894

Citation	Léger-Silvestre, I, Milkereit, P, Ferreira-Cerca, S, Saveanu, C, Rousselle, JC, Choesmel, V, Guinefoleau, C, Gas, N and Gleizes, PE (2004) The ribosomal protein Rps15p is required for nuclear exit of the 40S subunit precursors in yeast. EMBO J. 23:2336-47
Abstract	We have conducted a genetic screen in order to identify ribosomal proteins of Saccharomyces cerevisiae involved in nuclear export of the small subunit precursors. This has led us to distinguish Rps15p as a protein dispensable for maturation of the pre-40S particles, but whose assembly into the pre-ribosomes is a prerequisite to their nuclear exit. Upon depletion of Rps15p, 20S pre-rRNA is released from the nucleolus and retained in the nucleus, without alteration of the pre-rRNA early cleavages. In contrast, Rps18p, which contacts Rps15p in the small subunit, is required upstream for pre-rRNA processing at site A2. Most pre-40S specific factors are correctly associated with the intermediate particles accumulating in the nucleus upon Rps15p depletion, except the late-binding proteins Tsr1p and Rio2p. Here we show that these two proteins are dispensable for nuclear exit; instead, they participate in 20S pre-rRNA processing in the cytoplasm. We conclude that, during the final maturation steps in the nucleus, incorporation of the ribosomal protein Rps15p is specifically required to render the pre-40S particles competent for translocation to the cytoplasm.
Links	PubMed PMC423291 Online version:10.1038/sj.emboj.7600252
Keywords	Cell Nucleus/metabolism; Nuclear Proteins/metabolism; Protein Transport; RNA Precursors/metabolism; RNA, Ribosomal/metabolism; Saccharomyces cerevisiae/metabolism
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