GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:15136153
| Citation |
Tutuncu, L, Stein, P, Ord, TS, Jorgez, CJ and Williams, CJ (2004) Calreticulin on the mouse egg surface mediates transmembrane signaling linked to cell cycle resumption. Dev. Biol. 270:246-60 |
|---|---|
| Abstract |
Calreticulin, a protein best known as an endoplasmic reticulum chaperone, also is found on the extracellular plasma membrane surface of many cell types where it serves as a mediator of adhesion and as a regulator of the immune response. In this report, we demonstrate that calreticulin is present on the extracellular surface of the mouse egg plasma membrane and is increased in the perivitelline space after egg activation. The extracellular calreticulin appears to be secreted by vesicles in the egg cortex that are distinct from cortical granules. An anticalreticulin antibody binds to extracellular calreticulin on live eggs and inhibits sperm-egg binding but not fusion. In addition, engagement of cell surface calreticulin by incubation of mouse eggs in the presence of anticalreticulin antibodies results in alterations in the localization of cortical actin and the resumption of meiosis as indicated by alterations in chromatin configuration, decreases in cdc2/cyclin B1 and MAP kinase activities, and pronuclear formation. These events occur in the absence of any observable alterations in intercellular calcium. These data demonstrate that calreticulin functionally interacts with the egg cytoskeleton and can mediate transmembrane signaling linked to cell cycle resumption. These studies suggest a role for calreticulin as a lectin that may be involved in signal transduction events during or after sperm-egg interactions at fertilization. |
| Links |
PubMed Online version:10.1016/j.ydbio.2004.02.008 |
| Keywords |
Actins/metabolism; Animals; Antibodies/metabolism; Calcium/metabolism; Calreticulin/immunology; Calreticulin/metabolism; Cell Cycle/physiology; Cell Membrane/metabolism; Cytoplasmic Vesicles/chemistry; Cytoplasmic Vesicles/metabolism; DNA/metabolism; Embryo, Mammalian/cytology; Embryo, Mammalian/physiology; Female; Fertilization in Vitro; Mice; Oocytes/cytology; Oocytes/physiology; Signal Transduction/physiology; Sperm-Ovum Interactions |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
