PMID:15133481

Citation	Pryor, PR, Mullock, BM, Bright, NA, Lindsay, MR, Gray, SR, Richardson, SC, Stewart, A, James, DE, Piper, RC and Luzio, JP (2004) Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events. EMBO Rep. 5:590-5
Abstract	Both heterotypic and homotypic fusion events are required to deliver endocytosed macromolecules to lysosomes and remodel late endocytic organelles. A trans-SNARE complex consisting of Q-SNAREs syntaxin 7, Vti1b and syntaxin 8 and the R-SNARE VAMP8 has been shown by others to be responsible for homotypic fusion of late endosomes. Using antibody inhibition experiments in rat liver cell-free systems, we confirmed this result, but found that the same Q-SNAREs can combine with an alternative R-SNARE, namely VAMP7, for heterotypic fusion between late endosomes and lysosomes. Co-immunoprecipitation demonstrated separate syntaxin 7 complexes with either VAMP7 or VAMP8 in solubilized rat liver membranes. Additionally, overexpression of the N-terminal domain of VAMP7, in cultured fibroblastic cells, inhibited the mixing of a preloaded lysosomal content marker with a marker delivered to late endosomes. These data show that combinatorial interactions of SNAREs determine whether late endosomes undergo homotypic or heterotypic fusion events.
Links	PubMed PMC1299070 Online version:10.1038/sj.embor.7400150
Keywords	Animals; Cell-Free System/immunology; Cell-Free System/metabolism; Endocytosis/physiology; Endosomes/immunology; Endosomes/metabolism; Immunoprecipitation; Lysosomes/immunology; Lysosomes/metabolism; Membrane Fusion; Membrane Proteins/immunology; Membrane Proteins/metabolism; PC12 Cells; Protein Transport/physiology; Qa-SNARE Proteins; R-SNARE Proteins; Rats; SNARE Proteins; Vesicular Transport Proteins/immunology; Vesicular Transport Proteins/metabolism
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.