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PMID:15123614
| Citation |
Höflich, J, Berninsone, P, Göbel, C, Gravato-Nobre, MJ, Libby, BJ, Darby, C, Politz, SM, Hodgkin, J, Hirschberg, CB and Baumeister, R (2004) Loss of srf-3-encoded nucleotide sugar transporter activity in Caenorhabditis elegans alters surface antigenicity and prevents bacterial adherence. J. Biol. Chem. 279:30440-8 |
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| Abstract |
During the establishment of a bacterial infection, the surface molecules of the host organism are of particular importance, since they mediate the first contact with the pathogen. In Caenorhabditis elegans, mutations in the srf-3 locus confer resistance to infection by Microbacterium nematophilum, and they also prevent biofilm formation by Yersinia pseudotuberculosis, a close relative of the bubonic plague agent Yersinia pestis. We cloned srf-3 and found that it encodes a multitransmembrane hydrophobic protein resembling nucleotide sugar transporters of the Golgi apparatus membrane. srf-3 is exclusively expressed in secretory cells, consistent with its proposed function in cuticle/surface modification. We demonstrate that SRF-3 can function as a nucleotide sugar transporter in heterologous in vitro and in vivo systems. UDP-galactose and UDP-N-acetylglucosamine are substrates for SRF-3. We propose that the inability of Yersinia biofilms and M. nematophilum to adhere to the nematode cuticle is due to an altered glycoconjugate surface composition of the srf-3 mutant. |
| Links |
PubMed Online version:10.1074/jbc.M402429200 |
| Keywords |
Amino Acid Sequence; Animals; Bacterial Adhesion; Biological Transport; Caenorhabditis elegans; Carbohydrate Metabolism; Cell Membrane/metabolism; Cloning, Molecular; DNA, Complementary/metabolism; Dogs; Dose-Response Relationship, Drug; Exons; Golgi Apparatus/metabolism; Green Fluorescent Proteins; Introns; Luminescent Proteins/metabolism; Membrane Transport Proteins/chemistry; Membrane Transport Proteins/physiology; Microscopy, Fluorescence; Models, Genetic; Molecular Sequence Data; Mutation; Phenotype; Plasmids/metabolism; Protein Structure, Tertiary; Ricin/pharmacology; Sequence Homology, Amino Acid; Transfection; Uridine Diphosphate Galactose/metabolism; Uridine Diphosphate N-Acetylglucosamine/metabolism; Yersinia pseudotuberculosis |
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Significance
Annotations
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