GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:15075292
Citation |
Chen, MH, Li, YJ, Kawakami, T, Xu, SM and Chuang, PT (2004) Palmitoylation is required for the production of a soluble multimeric Hedgehog protein complex and long-range signaling in vertebrates. Genes Dev. 18:641-59 |
---|---|
Abstract |
Hedgehog (Hh) signaling plays a major role in multiple aspects of embryonic development. A key issue in Hh signaling is to elucidate the molecular mechanism by which a Hh protein morphogen gradient is formed despite its membrane association. In this study, we used a combination of genetic, cellular, and biochemical approaches to address the role of lipid modifications in long-range vertebrate Hh signaling. Our molecular analysis of knockout mice deficient in Skn, the murine homolog of the Drosophila ski gene, which catalyzes Hh palmitoylation, and gene-targeted mice producing a nonpalmitoylated form of Shh indicates that Hh palmitoylation is essential for its activity as well as the generation of a protein gradient in the developing embryos. Furthermore, our biochemical data show that Hh lipid modifications are required for producing a soluble multimeric protein complex, which constitutes the major active component for Hh signaling. These results suggest that soluble Hh multimeric complex travels in the morphogenetic field to activate Hh signaling in distant Hh-responsive cells. |
Links |
PubMed PMC387240 Online version:10.1101/gad.1185804 |
Keywords |
Animals; Hedgehog Proteins; Membrane Microdomains/metabolism; Mice; Mice, Transgenic; Signal Transduction/physiology; Trans-Activators/biosynthesis; Trans-Activators/metabolism |
edit table |
Significance
Annotations
Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
edit table |
See also
References
See Help:References for how to manage references in GONUTS.