GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Heath, V, Suh, HC, Holman, M, Renn, K, Gooya, JM, Parkin, S, Klarmann, KD, Ortiz, M, Johnson, P and Keller, J (2004) C/EBPalpha deficiency results in hyperproliferation of hematopoietic progenitor cells and disrupts macrophage development in vitro and in vivo. Blood 104:1639-47
CCAAT enhancer binding protein-alpha (C/EBPalpha) inhibits proliferation in multiple cell types; therefore, we evaluated whether C/EBPalpha-deficient hematopoietic progenitor cells (HPCs) have an increased proliferative potential in vitro and in vivo. In this study we demonstrate that C/EBPalpha(-/-) fetal liver (FL) progenitors are hyperproliferative, show decreased differentiation potential, and show increased self-renewal capacity in response to hematopoietic growth factors (HGFs). There are fewer committed bipotential progenitors in C/EBPalpha(-/-) FL, whereas multipotential progenitors are unaffected. HGF-dependent progenitor cell lines can be derived by directly culturing C/EBPalpha(-/-) FL cells in vitro Hyperproliferative spleen colonies and myelodysplastic syndrome (MDS) are observed in mice reconstituted with C/EBPalpha(-/-) FL cells, indicating progenitor hyperproliferation in vitro and in vivo. C/EBPalpha(-/-) FL lacked macrophage progenitors in vitro and had impaired ability to generate macrophages in vivo. These findings show that C/EBPalpha deficiency results in hyperproliferation of HPCs and a block in the ability of multipotential progenitors to differentiate into bipotential granulocyte/macrophage progenitors and their progeny.
Animals; CCAAT-Enhancer-Binding Protein-alpha/deficiency; CCAAT-Enhancer-Binding Protein-alpha/genetics; CCAAT-Enhancer-Binding Protein-alpha/metabolism; Cell Differentiation/drug effects; Cell Division/drug effects; Cell Transplantation; Cells, Cultured; Gene Deletion; Gene Expression Regulation, Developmental; Hematopoietic Stem Cells/drug effects; Hematopoietic Stem Cells/metabolism; Hematopoietic Stem Cells/pathology; Hepatocyte Growth Factor/pharmacology; Interleukin-3/metabolism; Liver/embryology; Liver/metabolism; Liver/pathology; Macrophages/drug effects; Macrophages/metabolism; Macrophages/pathology; Mice; Mice, Knockout; Proto-Oncogene Proteins c-met/genetics; Proto-Oncogene Proteins c-met/metabolism; Survival Rate
|Gene product||Qualifier||GO ID||GO term name||Evidence Code||with/from||Aspect||Notes||Status|
See Help:References for how to manage references in GONUTS.