PMID:15033264

Citation	Bourdineaud, JP, Nehmé, B, Tesse, S and Lonvaud-Funel, A (2004) A bacterial gene homologous to ABC transporters protect Oenococcus oeni from ethanol and other stress factors in wine. Int. J. Food Microbiol. 92:1-14
Abstract	The wine lactic acid bacteria Oenococcus oeni has to cope with harsh environmental conditions including an acidic pH, a high alcoholic content, non-optimal growth temperatures, and growth inhibitory compounds such as fatty acids, phenolic acids and tannins. We here describe characterisation and cloning of the O. oeni omrA gene encoding a protein belonging to the ATP-binding cassette superfamily of transporters. The OmrA protein displays the highest sequence similarity with the subfamily of ATP-dependent multidrug resistance (MDR) proteins, most notably the bacterial Lactococcus lactis LmrA homologue of the human MDR1 P-glycoprotein. The omrA gene proved to be a stress-responsive gene since its expression was increased at high temperature or under osmotic shock. The OmrA protein function was tested in Escherichia coli, and consistent with the omrA gene expression pattern, OmrA conferred protection to bacteria grown on a high salt medium. OmrA also triggered bacterial resistance to sodium laurate, wine and ethanol toxicity. The homologous LmrA protein featured the same stress-protective pattern than OmrA when expressed in E. coli, and the contribution to resistance of both OmrA and LmrA transporters was decreased by verapamil, a well-known inhibitor of the human MDR1 protein. Genes homologous to omrA were detected in other wine lactic acid bacteria, suggesting that this type of genes might constitute a well-conserved stress-protective molecular device.
Links	PubMed Online version:10.1016/S0168-1605(03)00162-4
Keywords	ATP-Binding Cassette Transporters/chemistry; ATP-Binding Cassette Transporters/genetics; Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Food Microbiology; Genes, Bacterial; Gram-Positive Cocci/genetics; Gram-Positive Cocci/growth & development; Gram-Positive Cocci/metabolism; Humans; Leuconostoc/genetics; Leuconostoc/growth & development; Leuconostoc/metabolism; Molecular Sequence Data; Multidrug Resistance-Associated Proteins/chemistry; Multidrug Resistance-Associated Proteins/genetics; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Wine/microbiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
OENOE:Q6X896		GO:0034605: cellular response to heat	ECO:0000270:		P		RT-PCR analysis showed that OmrA was produced in greater amounts of after heat-shock. (Figure-3)	complete
OENOE:Q6X896		GO:0071361: cellular response to ethanol	ECO:0000315:		P		Led to increased survival in wine/ethanol in transformed E. coli cells (Figure 5)	complete
OENOE:Q6X896		GO:0071236: cellular response to antibiotic	ECO:0000250:	UniProtKB:Q9CHL8	P		Comparison of the amino acid sequence showed a good deal of homology (59%) with the previously identified MDR1 protein LmrA. While homology was lower in the trans-membrane portions (395, the ABC domain involved efflux showed 72% similarity.	complete
OENOE:Q6X896		GO:0071475: cellular hyperosmotic salinity response	ECO:0000315:		P		The gene led to greater growth on a high salt medium on transformed E. coli cells (Figure 4)	complete

References

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