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PMID:14752161
| Citation |
Pal-Bhadra, M, Leibovitch, BA, Gandhi, SG, Rao, M, Bhadra, U, Birchler, JA and Elgin, SC (2004) Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery. Science 303:669-72 |
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| Abstract |
Genes normally resident in euchromatic domains are silenced when packaged into heterochromatin, as exemplified in Drosophila melanogaster by position effect variegation (PEV). Loss-of-function mutations resulting in suppression of PEV have identified critical components of heterochromatin, including proteins HP1, HP2, and histone H3 lysine 9 methyltransferase. Here, we demonstrate that this silencing is dependent on the RNA interference machinery, using tandem mini-white arrays and white transgenes in heterochromatin to show loss of silencing as a result of mutations in piwi, aubergine, or spindle-E (homeless), which encode RNAi components. These mutations result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2, most dramatically in spindle-E mutants. |
| Links |
PubMed Online version:10.1126/science.1092653 |
| Keywords |
ATP-Binding Cassette Transporters/genetics; ATP-Binding Cassette Transporters/physiology; Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/physiology; Alleles; Animals; Argonaute Proteins; Chromosomal Proteins, Non-Histone/genetics; Chromosomal Proteins, Non-Histone/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila Proteins/physiology; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Eye Proteins/genetics; Eye Proteins/physiology; Gene Silencing; Genes, Insect; Heterochromatin/metabolism; Histone-Lysine N-Methyltransferase/genetics; Histone-Lysine N-Methyltransferase/metabolism; Histones/metabolism; Methylation; Mutation; Proteins/genetics; Proteins/physiology; RNA Interference; RNA-Induced Silencing Complex; Transgenes |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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