PMID:14730308

Citation	Akum, BF, Chen, M, Gunderson, SI, Riefler, GM, Scerri-Hansen, MM and Firestein, BL (2004) Cypin regulates dendrite patterning in hippocampal neurons by promoting microtubule assembly. Nat. Neurosci. 7:145-52
Abstract	Dendrite branching has an important role in normal brain function. Here we report that overexpression of cypin, a protein that has guanine deaminase activity and is expressed in developing processes in rat hippocampal neurons, results in increased dendrite branching in primary culture. Mutant cypin proteins that lack guanine deaminase activity act in a dominant-negative manner when expressed in primary neurons. Furthermore, we knocked down cypin protein levels using a new strategy: expressing a 5' end-mutated U1 small nuclear RNA (snRNA) to inhibit maturation of cypin mRNA. Neurons that express this mutant snRNA show little or no detectable cypin protein and fewer dendrites than normal. In addition, we found that cypin binds directly to tubulin heterodimers and promotes microtubule polymerization. Thus, our results demonstrate a new pathway by which dendrite patterning is regulated, and we also introduce a new method for decreasing endogenous protein expression in neurons.
Links	PubMed Online version:10.1038/nn1179
Keywords	Animals; Body Patterning/physiology; Carrier Proteins/physiology; Cells, Cultured; Dendrites/physiology; Embryo, Mammalian; Guanine Deaminase/physiology; Hippocampus/embryology; Immunohistochemistry; Microtubules/physiology; Mutation; RNA, Small Nuclear/genetics; Rats; Transfection
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:GUAD		GO:0031116: positive regulation of microtubule polymerization	ECO:0000314:		P		Figure 7d shows that cypin (GDA) promotes microtubule polymerization.	complete CACAO 6377
RAT:GUAD	involved_in	GO:0031116: positive regulation of microtubule polymerization	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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