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PMID:14662849
| Citation |
Kretschmer, K, Jungebloud, A, Stopkowicz, J, Kleinke, T, Hoffmann, R and Weiss, S (2003) The selection of marginal zone B cells differs from that of B-1a cells. J. Immunol. 171:6495-501 |
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| Abstract |
Transgenic (Tg) L2 mice expressing high levels of the lambda2 (315) L chain contain only B cell populations involved in the first line of defense, i.e., B-1 and marginal zone (MZ) B cells. The strongly oligoclonal IgH chain repertoire of Tg B-1a cells in such mice was attributed to strong positive selection by autoantigens. In this study, we show that the MZ B cells of L2 mice correspond very closely to MZ B cells of normal mice, as revealed by surface marker expression and gene expression profiling. We demonstrate that the IgH chain repertoire of these Tg MZ B cells is extremely heterogeneous. This is in sharp contrast to the oligoclonality found in B-1a cells of the same mice, which was attributed to strong positive selection mediated by autoantigens. Therefore, the strong positive selection of the IgH chain repertoire in L2 mice is B-1a specific. Thus, our data demonstrate that despite common functional properties, MZ B and B-1a cells exhibit striking differences in their selection and/or maintenance requirements. |
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| Keywords |
Animals; B-Lymphocyte Subsets/cytology; B-Lymphocyte Subsets/immunology; B-Lymphocyte Subsets/metabolism; B-Lymphocyte Subsets/pathology; Bone Marrow Cells/cytology; Bone Marrow Cells/immunology; Bone Marrow Cells/metabolism; Bone Marrow Cells/pathology; Cell Differentiation/genetics; Cell Differentiation/immunology; Gene Expression Profiling; Gene Rearrangement, B-Lymphocyte, Heavy Chain; Immunoglobulin Heavy Chains/biosynthesis; Immunoglobulin Heavy Chains/genetics; Immunoglobulin Light Chains/biosynthesis; Immunoglobulin Light Chains/genetics; Immunoglobulin mu-Chains/biosynthesis; Immunoglobulin mu-Chains/genetics; Mice; Mice, Inbred BALB C; Mice, Transgenic; Molecular Sequence Data; Peritoneum/immunology; Peritoneum/metabolism; Peritoneum/pathology; Receptors, Complement 3d/biosynthesis; Receptors, IgE/biosynthesis; Spleen/immunology; Spleen/metabolism; Spleen/pathology |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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