PMID:1464311

Citation	Leberer, E, Dignard, D, Harcus, D, Thomas, DY and Whiteway, M (1992) The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components. EMBO J. 11:4815-24
Abstract	In the yeast Saccharomyces cerevisiae the G-protein beta gamma subunits have been shown to trigger downstream events of the pheromone response pathway. We have identified a new gene, designated STE20, which encodes a protein kinase homologue with sequence similarity to protein kinase C, which is required to transmit the pheromone signal from G beta gamma to downstream components of the signalling pathway. Overproduction of the kinase suppresses the mating defect of dominant-negative G beta mutations providing genetic evidence for an interaction with G beta, and epistasis experiments show that this kinase functions after or at the same point as G beta gamma, but before any of the other currently identified components of the signalling pathway. This points to a potentially new mechanism of G-protein mediated signal transduction, the activation of a protein kinase through G beta gamma.
Links	PubMed PMC556957
Keywords	Amino Acid Sequence; Base Sequence; Blotting, Northern; Chromosome Mapping; DNA, Fungal/metabolism; Fungal Proteins/genetics; Fungal Proteins/metabolism; GTP-Binding Proteins/metabolism; Intracellular Signaling Peptides and Proteins; Molecular Sequence Data; Phenotype; Pheromones/metabolism; Plasmids; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; RNA, Fungal/metabolism; Restriction Mapping; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Signal Transduction
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