PMID:14609575

Citation	Matsuzaki, J, Tsuji, T, Chamoto, K, Takeshima, T, Sendo, F and Nishimura, T (2003) Successful elimination of memory-type CD8+ T cell subsets by the administration of anti-Gr-1 monoclonal antibody in vivo. Cell. Immunol. 224:98-105
Abstract	During investigating the expression of Gr-1 antigen on various subsets of mouse spleen cells, we found that Gr-1 was expressed on memory-type CD8(+)CD44(high)CD62L(high) T cells in addition to granulocytes. Intraperitoneal administration of anti-Gr-1 mAb caused almost complete elimination of Ly-6C(+) memory-type CD8(+) T cells as well as Ly-6G(+) granulocytes. Anti-Gr-1 mAb-treated mouse spleen cells exhibited greatly reduced IFN-gamma production in response to anti-CD3 mAb both in vitro and in vivo. This reduced cytokine production appeared to be derived from elimination of IFN-gamma-producing Gr-1(+)CD8(+) T cells. Indeed, CD8(+) T cells with IFN-gamma-producing activity and cytotoxicity were generated from isolated Gr-1(+)CD8(+) cells but not from Gr-1(-)CD8(+) T cells. We also demonstrated that therapeutic effect of MBL-2 tumor-immunized spleen cells was greatly reduced by anti-Gr-1 mAb-treatment. Thus, we initially demonstrated that anti-Gr-1 mAb might become a good tool to investigate a precise role for memory-type CD8(+) T cells in vivo.
Links	PubMed
Keywords	Animals; Antibodies, Monoclonal/immunology; Antigens, CD3/immunology; Antigens, Differentiation, Myelomonocytic/analysis; Antigens, Differentiation, Myelomonocytic/physiology; Antigens, Ly/analysis; CD8-Positive T-Lymphocytes/immunology; Female; Immunization; Immunologic Memory; Immunotherapy, Adoptive; Interferon-gamma/biosynthesis; Lymphocyte Depletion; Lymphoma/immunology; Mice; Mice, Inbred C57BL; Neutrophils/physiology
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.