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PMID:14595833
| Citation |
Shentu, H, Wen, HJ, Her, GM, Huang, CJ, Wu, JL and Hwang, SP (2003) Proximal upstream region of zebrafish bone morphogenetic protein 4 promoter directs heart expression of green fluorescent protein. Genesis 37:103-12 |
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| Abstract |
We examined the activity of the bone morphogenetic protein 4 (BMP4) promoter in zebrafish embryos via transient and stable transgenic expression analyses in order to obtain a better understanding of the regulation of BMP4 tissue-specific expression. Transient expression studies showed that the 9.0-kb BMP4 promoter/upstream region drove green fluorescent protein (GFP) expression mainly in the heart. Deletion analyses indicated the existence of multiple regulatory elements in the 7.5-kb BMP4 promoter/proximal upstream region. In addition, a coinjection experiment further demonstrated the 2.4-kb Bgl II-Hind III DNA region contains major positive regulatory elements. In addition, stable transgenic lines were established to further confirm the heart-specificity of this segment in BMP4 promoter. The results showed that GFP was mainly localized in the myocardium of developing ventricles of 48-hpf (hours postfertilization), 72-hpf, and 100-hpf transgenic F(1) embryos. Together, these results indicate that the 7.5-kb BMP4 promoter/proximal upstream region specifically contains regulatory elements for BMP4 expression in the heart, while regulatory elements for other endogenous BMP4-expressing tissues may reside in more distal regions and/or in introns. |
| Links |
PubMed Online version:10.1002/gene.10240 |
| Keywords |
Animals; Bone Morphogenetic Protein 4; Bone Morphogenetic Proteins/genetics; Embryonic Development; Gene Expression Regulation, Developmental; Green Fluorescent Proteins; Heart/physiology; Indicators and Reagents/analysis; Luminescent Proteins/analysis; Luminescent Proteins/biosynthesis; Promoter Regions, Genetic; Zebrafish/embryology; Zebrafish/genetics; Zebrafish Proteins |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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