PMID:14561875

Citation	Yuan, A, Rao, MV, Kumar, A, Julien, JP and Nixon, RA (2003) Neurofilament transport in vivo minimally requires hetero-oligomer formation. J. Neurosci. 23:9452-8
Abstract	Neurofilament assembly requires at minimum the polymerization of neurofilament light chain (NF-L) with either neurofilament medium chain (NF-M) or neurofilament heavy chain (NF-H) subunits, but requirements for their axonal transport have long been controversial. Using a gene deletion approach, we generated mice containing only NF-L or NF-M. In vivo pulse radiolabeling analyses in retinal ganglion cell neurons revealed that NF-L alone is incapable of efficient transport, whereas nearly one-half of the normal level of NF-M is transported along optic axons in the absence of the other triplet subunits. Under these conditions, however, NF-M transport is completely abolished by deleting alpha-internexin. Our results strongly suggest that efficient neurofilament protein transport in vivo minimally requires hetero-oligomer formation. They also show that NF-M can partner with intermediate filament proteins other than the NF-H and NF-L subunits in neurons to support slow transport and possibly other functions of neuronal intermediate filaments.
Links	PubMed
Keywords	Animals; Axonal Transport/physiology; Axons/metabolism; Axons/ultrastructure; Carrier Proteins/genetics; Carrier Proteins/metabolism; Female; Intermediate Filament Proteins; Intermediate Filaments/metabolism; Intermediate Filaments/ultrastructure; Macromolecular Substances; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Neurofilament Proteins/deficiency; Neurofilament Proteins/genetics; Neurofilament Proteins/metabolism; Protein Subunits/metabolism; Protein Transport/physiology
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.