GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:14560010
| Citation |
Adhikary, S, Peukert, K, Karsunky, H, Beuger, V, Lutz, W, Elsässer, HP, Möröy, T and Eilers, M (2003) Miz1 is required for early embryonic development during gastrulation. Mol. Cell. Biol. 23:7648-57 |
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| Abstract |
Miz1 is a member of the POZ domain/zinc finger transcription factor family. In vivo, Miz1 forms a complex with the Myc oncoprotein and recruits Myc to core promoter elements. Myc represses transcription through Miz1 binding sites. We now show that the Miz1 gene is ubiquitously expressed during mouse embryogenesis. In order to elucidate the physiological function of Miz1, we have deleted the mouse Miz1 gene by homologous recombination. Miz1(+/-) mice are indistinguishable from wild-type animals; in contrast, Miz1(-/-) embryos are not viable. They are severely retarded in early embryonic development and do not undergo normal gastrulation. Expression of Goosecoid and Brachyury is detectable in Miz1(-/-) embryos, suggesting that Miz1 is not required for signal transduction by Nodal. Expression of p21Cip1, a target gene of Miz1 is unaltered; in contrast, expression of p57Kip2, another target gene of Miz1 is absent in Miz1(-/-) embryos. Miz1(-/-) embryos succumb to massive apoptosis of ectodermal cells around day 7.5 of embryonic development. Our results show that Miz1 is required for early embryonic development during gastrulation. |
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| Keywords |
Animals; Apoptosis/physiology; Cell Cycle Proteins/metabolism; Cell Division/physiology; Cyclin-Dependent Kinase Inhibitor p15; Cyclin-Dependent Kinase Inhibitor p16/metabolism; Cyclin-Dependent Kinase Inhibitor p21; Cyclin-Dependent Kinase Inhibitor p57; Cyclins/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Enzyme Inhibitors/metabolism; Female; Fetal Proteins; Gastrula/cytology; Gastrula/physiology; Gene Expression Regulation, Developmental; Gene Targeting; Goosecoid Protein; Homeodomain Proteins/metabolism; Humans; In Situ Hybridization; Kruppel-Like Transcription Factors; Mesoderm/physiology; Mice; Mice, Inbred C57BL; Mice, Knockout; Morphogenesis; Nuclear Proteins/metabolism; Phenotype; Proto-Oncogene Proteins c-myc/genetics; Proto-Oncogene Proteins c-myc/metabolism; Repressor Proteins; T-Box Domain Proteins/metabolism; Transcription Factors; Transcription, Genetic; Tumor Suppressor Proteins |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
References
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