GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:14551328
| Citation |
Rhee, SY, Osborne, E, Poindexter, PD and Somerville, CR (2003) Microspore separation in the quartet 3 mutants of Arabidopsis is impaired by a defect in a developmentally regulated polygalacturonase required for pollen mother cell wall degradation. Plant Physiol. 133:1170-80 |
|---|---|
| Abstract |
Mutations in the QUARTET loci in Arabidopsis result in failure of microspore separation during pollen development due to a defect in degradation of the pollen mother cell wall during late stages of pollen development. Mutations in a new locus required for microspore separation, QRT3, were isolated, and the corresponding gene was cloned by T-DNA tagging. QRT3 encodes a protein that is approximately 30% similar to an endopolygalacturonase from peach (Prunus persica). The QRT3 protein was expressed in yeast (Saccharomyces cerevisiae) and found to exhibit polygalacturonase activity. In situ hybridization experiments showed that QRT3 is specifically and transiently expressed in the tapetum during the phase when microspores separate from their meiotic siblings. Immunohistochemical localization of QRT3 indicated that the protein is secreted from tapetal cells during the early microspore stage. Thus, QRT3 plays a direct role in degrading the pollen mother cell wall during microspore development. |
| Links |
PubMed PMC281612 Online version:10.1104/pp.103.028266 |
| Keywords |
Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Wall/metabolism; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genetic Complementation Test; Immunohistochemistry; Microscopy, Electron, Scanning; Molecular Sequence Data; Mutation; Pollen/enzymology; Pollen/genetics; Pollen/ultrastructure; Polygalacturonase/genetics; Polygalacturonase/metabolism; Prunus/enzymology; Prunus/genetics; Sequence Homology, Amino Acid |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
