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PMID:14535887
| Citation |
Apse, MP, Sottosanto, JB and Blumwald, E (2003) Vacuolar cation/H+ exchange, ion homeostasis, and leaf development are altered in a T-DNA insertional mutant of AtNHX1, the Arabidopsis vacuolar Na+/H+ antiporter. Plant J. 36:229-39 |
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| Abstract |
The function of vacuolar Na+/H+ antiporter(s) in plants has been studied primarily in the context of salinity tolerance. By facilitating the accumulation of Na+ away from the cytosol, plant cells can avert ion toxicity and also utilize vacuolar Na+ as osmoticum to maintain turgor. As many genes encoding these antiporters have been cloned from salt-sensitive plants, it is likely that they function in some capacity other than salinity tolerance. The wide expression pattern of Arabidopsis thaliana sodium proton exchanger 1 (AtNHX1) in this study supports this hypothesis. Here, we report the isolation of a T-DNA insertional mutant of AtNHX1, a vacuolar Na+/H+ antiporter in Arabidopsis. Vacuoles isolated from leaves of the nhx1 plants had a much lower Na+/H+ and K+/H+ exchange activity. nhx1 plants also showed an altered leaf development, with reduction in the frequency of large epidermal cells and a reduction in overall leaf area compared to wild-type plants. The overexpression of AtNHX1 in the nhx1 background complemented these phenotypes. In the presence of NaCl, nhx1 seedling establishment was impaired. These results place AtNHX1 as the dominant K+ and Na+/H+ antiporter in leaf vacuoles in Arabidopsis and also suggest that its contribution to ion homeostasis is important for not only salinity tolerance but development as well. |
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| Keywords |
Arabidopsis/genetics; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cation Transport Proteins/genetics; Cation Transport Proteins/metabolism; DNA, Bacterial/genetics; Gene Expression Regulation, Plant; Genetic Complementation Test; Mutagenesis, Insertional; Plant Epidermis/genetics; Plant Epidermis/physiology; Plant Leaves/genetics; Plant Leaves/physiology; Sodium-Hydrogen Antiporter/genetics; Sodium-Hydrogen Antiporter/metabolism |
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Significance
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