PMID:14504659

Citation	Hu, QD, Lu, H, Huo, K, Ying, K, Li, J, Xie, Y, Mao, Y and Li, YY (2003) A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis. Cell. Mol. Life Sci. 60:1725-32
Abstract	The Saccharomyces cerevisiae TPT1 gene plays a role in removing the 2'-phosphate from ligated tRNA during the maturation of pre-tRNA. Here we reported the cloning and characterization of the human TRPT1 gene as a homolog of yeast TPT1. The TRPT1 gene is located at human chromosome 11q13 and encodes a polypeptide of 253 amino acids. BLAST searches with its amino acid sequence revealed the ubiquitous occurrence of TRPT1 homologs and their functional relationships with the presence of the DUF60/KptA domain. Northern analysis demonstrated that the gene is primarily expressed in heart and skeletal muscle, with lower or undetectable levels in other tissues studied. A plasmid-shuffling experiment showed that the human TRPT1 gene could complement the tpt1 mutation in S. cerevisiae
Links	PubMed Online version:10.1007/s00018-003-3107-7
Keywords	Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA, Complementary/genetics; Exons; Gene Expression; Genes, Fungal; Genetic Complementation Test; Humans; Introns; Molecular Sequence Data; Mutation; Phosphotransferases (Alcohol Group Acceptor)/chemistry; Phosphotransferases (Alcohol Group Acceptor)/genetics; Phosphotransferases (Alcohol Group Acceptor)/metabolism; Plasmids/genetics; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Species Specificity
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