PMID:1439823

Citation	Hendrix, RW and Duda, RL (1992) Bacteriophage lambda PaPa: not the mother of all lambda phages. Science 258:1145-8
Abstract	The common laboratory strain of bacteriophage lambda--lambda wild type or lambda PaPa--carries a frameshift mutation relative to Ur-lambda, the original isolate. The Ur-lambda virions have thin, jointed tail fibers that are absent from lambda wild type. Two novel proteins of Ur-lambda constitute the fibers: the product of stf, the gene that is disrupted in lambda wild type by the frameshift mutation, and the product of gene tfa, a protein that is implicated in facilitating tail fiber assembly. Relative to lambda wild type, Ur-lambda has expanded receptor specificity and adsorbs to Escherichia coli cells more rapidly.
Links	PubMed
Keywords	Adsorption; Amino Acid Sequence; Bacteriophage lambda/chemistry; Bacteriophage lambda/genetics; Bacteriophage lambda/physiology; Chromosome Mapping; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli/radiation effects; Frameshift Mutation; Genes, Viral; Microscopy, Electron; Molecular Sequence Data; Molecular Weight; Ultraviolet Rays; Viral Tail Proteins/chemistry; Viral Tail Proteins/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
LAMBD:FIBER		GO:0098024: virus tail, fiber	ECO:0007044:		C		Fig. 3 shows that gene product stf produces a band on an SDS gel at approximately 78 kDa. Fig. 1 shows an electron microscopy image of Ur - lambda. Tail fibers are visible on the phage tail. The combine evidence shows that the stf gene product results in tail fiber formation at the virion tail. Organism: Escherichia phage lambda	complete CACAO 13518
LAMBD:FIBER		GO:0019062: virion attachment to host cell	ECO:0007040:		P		Fig. 4 demonstrates that Ur-lambda, which contains the tail fiber protein (stf), adsorbed to E. coli substantially faster than wild type lambda, which did not contain tail fiber proteins. Ur-lambda was capable of adsorbing in the presence of glucose, while wild type lambda was only capable of adsorbing in the presence of maltose. This evidence shows that the tail fiber proteins are involved in adsorption to the host cell. Organism: Escherichia phage lambda	complete CACAO 13522

References

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