PMID:1433508

Citation	Guarino, LA, Dong, W, Xu, B, Broussard, DR, Davis, RW and Jarvis, DL (1992) Baculovirus phosphoprotein pp31 is associated with virogenic stroma. J. Virol. 66:7113-20
Abstract	The PstI K fragment of Autographa californica nuclear polyhedrosis virus (AcMNPV) encodes a protein with a molecular weight of 31,000. To define the role of this protein (pp31) in virus infection further, it was overexpressed in bacteria and used to produce polyclonal antiserum. Radioimmunoprecipitation analysis indicated that pp31 was synthesized during both the early and late phases of virus infection, consistent with previous analyses indicating that the gene was regulated by tandem early and late promoters. Metabolic labeling of cells with carrier-free phosphate indicated that pp31 was phosphorylated. Biochemical fractionation experiments showed that pp31 was localized in the nucleus and that it was more stably associated with the nucleus at later times of infection. Immunoblot analysis of subnuclear fractions indicated that pp31 was associated predominantly with the chromatin and nuclear matrix fractions. Immunofluorescence experiments confirmed that the pp31 protein was localized in the nucleus. Nuclear staining was relatively uniform early but was more centrally nuclear later in infection. Immunoelectron microscopy indicated that the pp31 protein was a component of virogenic stroma. Southwestern (DNA-protein) blot analysis demonstrated that pp31 is a DNA-binding protein. These findings suggest a possible role for pp31 in the virus life cycle.
Links	PubMed PMC240392
Keywords	Animals; Baculoviridae/genetics; Baculoviridae/metabolism; Base Sequence; Cell Line; Cell Nucleus/metabolism; Cloning, Molecular; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Immunoblotting; Immunohistochemistry; Insects; Microscopy, Immunoelectron; Molecular Sequence Data; Molecular Weight; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Phosphoproteins/genetics; Phosphoproteins/metabolism; Promoter Regions, Genetic; Restriction Mapping
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
NPVAC:PP31		GO:0042025: host cell nucleus	ECO:0000314:		C		Organism: Autographa californica nuclear polyhedrosis virus Protein: pp31//pp31 Fig. 1 shows the nucleic and cytoplasmic fractions and shows the protein localized in the host cell nucleus fractions.	complete CACAO 12540
NPVAC:PP31		GO:0039720: virogenic stroma	ECO:0000314:		C		Organism: Autographa californica nuclear polyhedrosis virus Protein: pp31//pp31 Figure 6 shows the anti-pp31 stain on the virogenic stroma indicating the involvement of pp31 in the formation of the virogenic stroma	complete CACAO 12541

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