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PMID:13838951

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Citation

TORRIANI, A (1960) Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim. Biophys. Acta 38:460-9

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Escherichia coli/metabolism; Phosphates/pharmacology; Phosphoric Monoester Hydrolases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLX:Q14F13

GO:0008152: metabolic process

ECO:0000314:

P

Figure 1 shows increasing yield of bacteria with increasing phosphate concentration. Alkaline phosphatase activity is shown to be necessary for growth in phosphate-limiting situations.

complete

ECOLX:Q14F13

GO:0016791: phosphatase activity

ECO:0000314:

F

Fig. 2 shows phosphatase activity as a function of pH, where activity is measured by micromoles of liberated p-nitrophenol when its phosphate is cleaved.

complete

ECOLX:Q14F13

GO:0016311: dephosphorylation

ECO:0000314:

P

When bacterial suspension at pH 8.8 is mixed with p-nitrophenylphosphate substrate, appearance of cleaved NP is followed directly at .42 micromoles.

complete


See also

References

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