PMID:1325967

Citation	Conlin, CA, Trun, NJ, Silhavy, TJ and Miller, CG (1992) Escherichia coli prlC encodes an endopeptidase and is homologous to the Salmonella typhimurium opdA gene. J. Bacteriol. 174:5881-7
Abstract	Mutations at the Escherichia coli prlC locus suppress the export defect of certain lamB signal sequence mutations. The Salmonella typhimurium opdA gene encodes an endoprotease that can participate in the catabolism of certain peptides and is required for normal development of phage P22. Plasmids carrying either the wild-type (pTC100 prlC+) or suppressor alleles of prlC complemented all phenotypes associated with an S. typhimurium opdA mutation. A plasmid carrying an amber mutation in prlC [prlC31(AM)] was unable to complement except in an amber suppressor background. Tn1000 insertions which eliminated the ability of pTC100 (prlC+) to complement opdA mapped to the region of the plasmid shown by deletion analysis and subcloning to contain prlC. The nucleotide sequence of a 2.7-kb fragment including this region was determined, revealing an open reading frame encoding a 77-kDa protein. The sequences of this open reading frame and its putative promoter region were very similar (84% nucleotide sequence identity and 95% amino acid identity) to those of S. typhimurium opdA, showing that these genes are homologs. The nucleotide sequence of the prlC1 suppressor allele was determined and predicts an in-frame duplication of seven amino acids, providing further confirmation that the prlC suppressor phenotype results from changes in the endopeptidase OpdA.
Links	PubMed PMC207123
Keywords	Alleles; Amino Acid Sequence; Base Sequence; Chromosome Mapping; DNA Transposable Elements; Endopeptidases/genetics; Endopeptidases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genetic Complementation Test; Metalloendopeptidases; Molecular Sequence Data; Multigene Family/genetics; Mutagenesis, Insertional; Salmonella typhimurium/enzymology; Salmonella typhimurium/genetics; Sequence Homology, Nucleic Acid
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