PMID:12975351

Citation	Rogers, SL, Wiedemann, U, Stuurman, N and Vale, RD (2003) Molecular requirements for actin-based lamella formation in Drosophila S2 cells. J. Cell Biol. 162:1079-88
Abstract	Cell migration occurs through the protrusion of the actin-enriched lamella. Here, we investigated the effects of RNAi depletion of approximately 90 proteins implicated in actin function on lamella formation in Drosophila S2 cells. Similar to in vitro reconstitution studies of actin-based Listeria movement, we find that lamellae formation requires a relatively small set of proteins that participate in actin nucleation (Arp2/3 and SCAR), barbed end capping (capping protein), filament depolymerization (cofilin and Aip1), and actin monomer binding (profilin and cyclase-associated protein). Lamellae are initiated by parallel and partially redundant signaling pathways involving Rac GTPases and the adaptor protein Nck, which stimulate SCAR, an Arp2/3 activator. We also show that RNAi of three proteins (kette, Abi, and Sra-1) known to copurify with and inhibit SCAR in vitro leads to SCAR degradation, revealing a novel function of this protein complex in SCAR stability. Our results have identified an essential set of proteins involved in actin dynamics during lamella formation in Drosophila S2 cells.
Links	PubMed PMC2172842 Online version:10.1083/jcb.200303023
Keywords	Actin Cytoskeleton/metabolism; Actin Cytoskeleton/ultrastructure; Actin Depolymerizing Factors; Actin-Related Protein 2; Actins/antagonists & inhibitors; Actins/deficiency; Actins/genetics; Actins/metabolism; Adaptor Proteins, Signal Transducing; Animals; Caenorhabditis elegans Proteins/antagonists & inhibitors; Caenorhabditis elegans Proteins/genetics; Carrier Proteins/antagonists & inhibitors; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Cycle Proteins/antagonists & inhibitors; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Line; Cell Movement/physiology; Contractile Proteins; Cytoskeletal Proteins/antagonists & inhibitors; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/metabolism; Destrin; Drosophila Proteins/antagonists & inhibitors; Drosophila Proteins/deficiency; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster; Eukaryotic Cells/cytology; Eukaryotic Cells/metabolism; Microfilament Proteins/antagonists & inhibitors; Microfilament Proteins/deficiency; Microfilament Proteins/genetics; Profilins; Pseudopodia/metabolism; Pseudopodia/ultrastructure; RNA Interference; rac GTP-Binding Proteins/metabolism
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.