PMID:12944392

Citation	Griffitts, JS, Huffman, DL, Whitacre, JL, Barrows, BD, Marroquin, LD, Müller, R, Brown, JR, Hennet, T, Esko, JD and Aroian, RV (2003) Resistance to a bacterial toxin is mediated by removal of a conserved glycosylation pathway required for toxin-host interactions. J. Biol. Chem. 278:45594-602
Abstract	Crystal (Cry) proteins made by the bacterium Bacillus thuringiensis are pore-forming toxins that specifically target insects and nematodes and are used around the world to kill insect pests. To better understand how pore-forming toxins interact with their host, we have screened for Caenorhabditis elegans mutants that resist Cry protein intoxication. We find that Cry toxin resistance involves the loss of two glycosyltransferase genes, bre-2 and bre-4. These glycosyltransferases function in the intestine to confer susceptibility to toxin. Furthermore, they are required for the interaction of active toxin with intestinal cells, suggesting they make an oligosaccharide receptor for toxin. Similarly, the bre-3 resistance gene is also required for toxin interaction with intestinal cells. Cloning of the bre-3 gene indicates it is the C. elegans homologue of the Drosophila egghead (egh) gene. This identification is striking given that the previously identified bre-5 has homology to Drosophila brainiac (brn) and that egh-brn likely function as consecutive glycosyltransferases in Drosophila epithelial cells. We find that, like in Drosophila, bre-3 and bre-5 act in a single pathway in C. elegans. bre-2 and bre-4 are also part of this pathway, thereby extending it. Consistent with its homology to brn, we demonstrate that C. elegans bre-5 rescues the Drosophila brn mutant and that BRE-5 encodes the dominant UDP-GlcNAc:Man GlcNAc transferase activity in C. elegans. Resistance to Cry toxins has uncovered a four component glycosylation pathway that is functionally conserved between nematodes and insects and that provides the basis of the dominant mechanism of resistance in C. elegans.
Links	PubMed Online version:10.1074/jbc.M308142200
Keywords	Amino Acid Sequence; Animals; Bacterial Proteins/pharmacology; Bacterial Toxins; Caenorhabditis elegans; Caenorhabditis elegans Proteins/chemistry; Caenorhabditis elegans Proteins/physiology; Cloning, Molecular; Cytoplasm/metabolism; Dose-Response Relationship, Drug; Drosophila; Drosophila Proteins; Drosophila melanogaster; Endocytosis; Endotoxins/pharmacology; Genetic Complementation Test; Glycosylation; Glycosyltransferases/chemistry; Glycosyltransferases/physiology; Hemolysin Proteins; Intestines/metabolism; Membrane Proteins/metabolism; Microscopy, Fluorescence; Models, Biological; Molecular Sequence Data; Mutation; N-Acetylglucosaminyltransferases/metabolism; Oligosaccharides/metabolism; Sequence Homology, Amino Acid
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