PMID:12771432

Citation	Finke, S, Mueller-Waldeck, R and Conzelmann, KK (2003) Rabies virus matrix protein regulates the balance of virus transcription and replication. J. Gen. Virol. 84:1613-21
Abstract	RNA synthesis by negative-strand RNA viruses (NSVs) involves transcription of subgenomic mRNAs and replication of ribonucleoprotein complexes. In this study, the envelope matrix (M) protein of rabies virus (RV) was identified as a factor which inhibits transcription and stimulates replication. Transcription, but not replication, of RV minigenomes or of full-length RV was decreased by expression of heterologous M. Since RV assembly involving M and the glycoprotein G renders virus synthetically quiescent, an RV was generated with the M and G genes substituted by placeholders. Surprisingly, RNA synthesis by this recombinant was characterized not only by an increased transcription rate but also by a diminished accumulation of replication products. This phenotype was reversed in a dose-dependent manner by providing M in trans, showing that M is a replication-stimulatory factor. The role of M in determining the balance of replication and transcription was further exploited by generation of a recombinant RV with attenuated M expression, which is highly active in transcription. Regulation of RNA synthesis by matrix proteins may represent a general mechanism of nonsegmented NSVs, which is probably obscured by the silencing activity of M during virus assembly.
Links	PubMed
Keywords	Animals; Cell Line; Cricetinae; Gene Expression; Gene Silencing; Genome, Viral; RNA, Viral/biosynthesis; RNA, Viral/genetics; Rabies virus/genetics; Rabies virus/physiology; Transcription, Genetic; Viral Matrix Proteins/genetics; Viral Matrix Proteins/physiology; Virus Replication
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RABVS:MATRX		GO:0016032: viral reproduction	ECO:0000314:		P		Figure 2 shows that adding extra M proteins resulted in less transcription replication still remained high.	complete CACAO 6326
RABVS:MATRX		GO:0032897: negative regulation of viral transcription	ECO:0000315:		P		Figure 2: Over expression of M in a RV infected cell yields a decrease in transcription, as shown in the Northern blot	complete CACAO 6420
RABVS:MATRX	involved_in	GO:0032897: negative regulation of viral transcription	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

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