PMID:12759182

Citation	Kim, H, Chang, DJ, Lee, JA, Lee, YS and Kaang, BK (2003) Identification of nuclear/nucleolar localization signal in Aplysia learning associated protein of slug with a molecular mass of 18 kDa homologous protein. Neurosci. Lett. 343:134-8
Abstract	We isolated a learning associated protein of slug with a molecular mass of 18 kDa (LAPS18) homologue from the expressed sequence tag database of Aplysia kurodai and named it Aplysia LAPS18-like protein (ApLLP). ApLLP encodes 120 amino acids and has 57% identity with LAPS18. To examine the subcellular expression pattern of ApLLP we constructed an EGFP-tagged ApLLP fusion protein and overexpressed it in both Aplysia neurons and COS-7 cells. In contrast to the previous findings, which showed that LAPS18 is secreted by COS-7 cells, ApLLP-EGFP was localized to the nucleus, and most of it to nucleoli. Analysis of deletion mutants of ApLLP-EGFP showed that the N-terminal and the C-terminal nucleolar and nucleus localization signal sequences are important for localization to the nucleus and the nucleoli.
Links	PubMed
Keywords	Animals; Aplysia/genetics; Base Sequence; Behavior, Animal; COS Cells; DNA, Complementary; Gene Expression; Green Fluorescent Proteins; Learning/physiology; Luminescent Proteins; Neurons/metabolism; Nuclear Localization Signals/chemistry; Nuclear Localization Signals/genetics; Polymerase Chain Reaction/methods; Proteins/physiology; Recombinant Fusion Proteins/biosynthesis; Sequence Alignment; Sequence Deletion; Sequence Homology, Amino Acid; Software; Subcellular Fractions/metabolism; Transfection; Viral Proteins/chemistry; Viral Proteins/genetics
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