GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:12756174
| Citation |
Bäumer, N, Marquardt, T, Stoykova, A, Spieler, D, Treichel, D, Ashery-Padan, R and Gruss, P (2003) Retinal pigmented epithelium determination requires the redundant activities of Pax2 and Pax6. Development 130:2903-15 |
|---|---|
| Abstract |
The transcription factors Pax2 and Pax6 are co-expressed in the entire optic vesicle (OV) prior and concomitant with the establishment of distinct neuroretinal, retinal, pigmented-epithelial and optic-stalk progenitor domains, suggesting redundant functions during retinal determination. Pax2; Pax6 compound mutants display a dose-dependent reduction in the expression of the melanocyte determinant Mitf, accompanied by transdifferentiation of retinal pigmented epithelium (RPE) into neuroretina (NR) in Pax2(-/-); Pax6(+/-) embryos, which strongly resembles the phenotype of Mitf-null mutants. In Pax2(-/-); Pax6(-/-) OVs Mitf fails to be expressed and NR markers occupy the area that usually represents the Mitf(+) RPE domain. Furthermore, both, Pax2 and Pax6 bind to and activate a MITF RPE-promoter element in vitro, whereas prolonged expression of Pax6 in the Pax2-positive optic stalk leads to ectopic Mitf expression and RPE differentiation in vivo. Together, these results demonstrate that the redundant activities of Pax2 and Pax6 direct the determination of RPE, potentially by directly controlling the expression of RPE determinants. |
| Links | |
| Keywords |
Animals; Biological Markers; Cell Differentiation/physiology; Cell Line; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Embryo, Mammalian/anatomy & histology; Embryo, Mammalian/physiology; Eye Proteins/genetics; Eye Proteins/metabolism; Gene Expression Regulation, Developmental; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; Humans; Immunohistochemistry; Mice; Mice, Transgenic; Microphthalmia-Associated Transcription Factor; PAX2 Transcription Factor; Paired Box Transcription Factors; Pigment Epithelium of Eye/cytology; Pigment Epithelium of Eye/embryology; Pigment Epithelium of Eye/growth & development; Pigment Epithelium of Eye/physiology; Promoter Regions, Genetic; Repressor Proteins/genetics; Repressor Proteins/metabolism; Stem Cells/cytology; Stem Cells/physiology; Transcription Factors/genetics; Transcription Factors/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
