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PMID:12743288

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Citation

Strömsten, NJ, Bamford, DH and Bamford, JK (2003) The unique vertex of bacterial virus PRD1 is connected to the viral internal membrane. J. Virol. 77:6314-21

Abstract

Icosahedral double-stranded DNA (dsDNA) bacterial viruses are known to package their genomes into preformed procapsids via a unique portal vertex. Bacteriophage PRD1 differs from the more commonly known icosahedral dsDNA phages in that it contains an internal lipid membrane. The packaging of PRD1 is known to proceed via preformed empty capsids. Now, a unique vertex has been shown to exist in PRD1. We show in this study that this unique vertex extends to the virus internal membrane via two integral membrane proteins, P20 and P22. These small membrane proteins are necessary for the binding of the putative packaging ATPase P9, via another capsid protein, P6, to the virus particle.

Links

PubMed PMC155016

Keywords

Bacteriophage PRD1/genetics; Bacteriophage PRD1/metabolism; Capsid/metabolism; Cell Line; Escherichia coli/virology; Intracellular Membranes/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mutation; Salmonella enterica/virology; Viral Structural Proteins/genetics; Viral Structural Proteins/metabolism; Virion/metabolism; Virion/ultrastructure; Virus Assembly

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BPPRD:P2

GO:0019012: virion

ECO:0000314:

C

Figure 3.

complete
CACAO 7499

BPPRD:P2

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:P5

GO:0019012: virion

ECO:0000314:

C

Figure 3.

complete
CACAO 7500

BPPRD:P5

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:VP31

GO:0019012: virion

ECO:0000314:

C

Figure 3.

complete
CACAO 7506

BPPRD:VP31

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:PKG6

GO:0019012: virion

ECO:0000314:

C

Figure 3.

complete
CACAO 7503

BPPRD:PKG6

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:PKG9

GO:0019012: virion

ECO:0000314:

C

Figure 3.

complete
CACAO 7505

BPPRD:PKG9

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:PKG22

GO:0019012: virion

ECO:0000314:

C

Figure 3.

complete
CACAO 7507

BPPRD:PKG22

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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