PMID:12702465

Citation	Stambuk, BU and de Araujo, PS (2001) Kinetics of active alpha-glucoside transport in Saccharomyces cerevisiae. FEMS Yeast Res. 1:73-8
Abstract	alpha-Glucosides are the most abundant fermentable sugars in the industrial applications of Saccharomyces cerevisiae, and the active transport across the plasma membrane is the rate-limiting step for their metabolism. In this report we performed a detailed kinetic analysis of the active alpha-glucoside transport system(s) present in a wild-type strain, and in strains with defined alpha-glucoside permeases. Our results indicate that the wild-type strain harbors active transporters with high and low affinity for maltose and trehalose, and low-affinity transport systems for maltotriose and alpha-methylglucoside. The maltose permease encoded by the MAL21 gene showed a high affinity (K(m) approximately 5 mM) for maltose, and a low affinity (K(m) approximately 90 mM) for trehalose. On the other hand, the alpha-glucoside permease encoded by the AGT1 gene had a high affinity (K(m) approximately 7 mM) for trehalose, a low affinity (K(m) approximately 18 mM) for maltose and maltotriose, and a very low affinity (K(m) approximately 35 mM) for alpha-methylglucoside.
Links	PubMed
Keywords	Biological Transport, Active; Culture Media; Gene Expression Regulation, Fungal; Glucosides/metabolism; Kinetics; Maltose/metabolism; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Methylglucosides/metabolism; Monosaccharide Transport Proteins/genetics; Monosaccharide Transport Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Symporters/genetics; Symporters/metabolism; Trehalose/metabolism; Trisaccharides/metabolism
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