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PMID:12644507

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Citation

Cantwell, BJ, Draheim, RR, Weart, RB, Nguyen, C, Stewart, RC and Manson, MD (2003) CheZ phosphatase localizes to chemoreceptor patches via CheA-short. J. Bacteriol. 185:2354-61

Abstract

We have investigated the conditions required for polar localization of the CheZ phosphatase by using a CheZ-green fluorescent protein fusion protein that, when expressed from a single gene in the chromosome, restored chemotaxis to a DeltacheZ strain. Localization was observed in wild-type, DeltacheZ, DeltacheYZ, and DeltacheRB cells but not in cells with cheA, cheW, or all chemoreceptor genes except aer deleted. Cells making only CheA-short (CheA(S)) or CheA lacking the P2 domain also retained normal localization, whereas cells producing only CheA-long or CheA missing the P1 and P2 domains did not. We conclude that CheZ localization requires the truncated C-terminal portion of the P1 domain present in CheA(S). Missense mutations targeting residues 83 through 120 of CheZ also abolished localization. Two of these mutations do not disrupt chemotaxis, indicating that they specifically prevent interaction with CheA(S) while leaving other activities of CheZ intact.

Links

PubMed PMC151485

Keywords

Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Chemoreceptor Cells/metabolism; Chemotaxis/genetics; Conserved Sequence; Enterobacteriaceae/metabolism; Green Fluorescent Proteins; Hydrophobic and Hydrophilic Interactions; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Models, Molecular; Molecular Sequence Data; Mutation; Protein Conformation; Protein Kinases/chemistry; Protein Kinases/genetics; Protein Kinases/metabolism; Protein Structure, Tertiary; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Sequence Homology, Amino Acid; Subcellular Fractions

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:CHEA

acts_upstream_of_or_within

GO:0051649: establishment of localization in cell

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:CHEA

involved_in

GO:0051649: establishment of localization in cell

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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