PMID:12638230

Citation	Kwon, J, Kikuchi, T, Setsuie, R, Ishii, Y, Kyuwa, S and Yoshikawa, Y (2003) Characterization of the testis in congenitally ubiquitin carboxy-terminal hydrolase-1 (Uch-L1) defective (gad) mice. Exp. Anim. 52:1-9
Abstract	The gracile axonal dystrophy (gad) mice are known to have a deletion within the gene encoding ubiquitin carboxy-terminal hydrolase-1 (Uch-L1) and show hereditary sensory deterioration and motor paresis. Expression of Uch-L1 is reported to be almost limited to the nervous system and testis. To understand whether Uch-L1, one of the major ubiquitin carboxy-terminal hydrolase (UCH) isozymes in the testis, affects spermatogenesis and other UCH isozymes (Uch-L3, L4 and L5) expression in the testis, we compared the testis between gad, hetero and wild type mice by histological, immunohistochemical analyses and RT-PCR. Histological analysis in 25-week-old gad mice showed shrinking of seminiferous tubules, decreasing total number of cells and enlargement of remaining cells in seminiferous tubules. By immunohistochemistry, a significant decrease (p < 0.05) in the number of proliferating cell nuclear antigen (PCNA) positive cells was observed. Expression of other UCH isozyme mRNAs was not apparently affected by Uch-L1 deficiency in 25-week-old gad mice. This study is the first report on the testis of gad mutant mouse.
Links	PubMed
Keywords	Animals; Atrophy; Gene Deletion; Immunohistochemistry; Isoenzymes/metabolism; Male; Mice; Mice, Mutant Strains; Proliferating Cell Nuclear Antigen/analysis; Seminiferous Tubules/cytology; Seminiferous Tubules/enzymology; Seminiferous Tubules/pathology; Thiolester Hydrolases/genetics; Thiolester Hydrolases/metabolism; Ubiquitin Thiolesterase
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