PMID:12582119

Citation	Wiatrowski, HA and Carlson, M (2003) Yap1 accumulates in the nucleus in response to carbon stress in Saccharomyces cerevisiae. Eukaryotic Cell 2:19-26
Abstract	Yap1 is a transcription factor of the AP-1 family that is required for the adaptive response to oxidative stress in Saccharomyces cerevisiae. We recovered Yap1 in a two-hybrid screen for proteins that interact with the Sip2 subunit of the Snf1 protein kinase, which is required for the adaptation of cells to glucose limitation. Yap1 becomes enriched in the nucleus when cells are subjected to oxidative stress. We show that the localization of Yap1 is similarly sensitive to carbon stress. When glucose-grown cells were shifted to medium containing glycerol or no added carbon source, green fluorescent protein (GFP)-Yap1 accumulated in the nucleus. After adaptation to growth in glycerol, GFP-Yap1 was again primarily cytoplasmic. Nuclear accumulation was independent of respiration and of the Snf1, PKA, TOR, and Yak1 pathways, and the mechanism is distinct from that involved in the response to hydrogen peroxide. Addition of glutathione to the medium inhibited nuclear accumulation of GFP-Yap1 in response to carbon stress but did not affect the relocalization of Gal83 or Mig1. Other stresses such as increased temperature, acidic pH, and ionic stress did not cause nuclear enrichment of GFP-Yap1. These findings suggest a role for Yap1 in the response to carbon stress.
Links	PubMed PMC141162
Keywords	Adaptation, Physiological/physiology; Carbon/deficiency; Cell Nucleus/genetics; Cell Nucleus/metabolism; Cell Respiration/genetics; Cyclic AMP-Dependent Protein Kinases/metabolism; DNA-Binding Proteins/metabolism; Glucose/deficiency; Glutathione/genetics; Glutathione/metabolism; Glycerol/metabolism; Glycerol/pharmacology; Intracellular Signaling Peptides and Proteins; Oxidative Stress/genetics; Phosphatidylinositol 3-Kinases/metabolism; Phosphotransferases (Alcohol Group Acceptor)/metabolism; Protein-Serine-Threonine Kinases/metabolism; Recombinant Fusion Proteins/diagnostic use; Repressor Proteins/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Up-Regulation/genetics
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