PMID:12514128

Citation	Foster, AJ, Jenkinson, JM and Talbot, NJ (2003) Trehalose synthesis and metabolism are required at different stages of plant infection by Magnaporthe grisea. EMBO J. 22:225-35
Abstract	The relationship of trehalose metabolism to fungal virulence was explored in the rice blast fungus Magnaporthe grisea. To determine the role of trehalose synthesis in pathogenesis, we identified and deleted TPS1, encoding trehalose-6-phosphate synthase. A Deltatps1 mutant failed to synthesize trehalose, sporulated poorly and was greatly attenuated in pathogenicity. Appressoria produced by Deltatps1 did not develop full turgor or elaborate penetration hyphae efficiently. To determine the role of subsequent trehalose breakdown, we deleted NTH1, which encodes a neutral trehalase. Nth1 mutants infected plants normally, but showed attenuated pathogenicity due to a decreased ability to colonize plant tissue. A second trehalase was also identified, required both for growth on trehalose and mobilization of intracellular trehalose during infection-related development. TRE1 encodes a cell wall-localized enzyme with characteristics of both neutral and acidic trehalases, but is dispensable for pathogenicity. Our results indicate that trehalose synthesis, but not its subsequent breakdown, is required for primary plant infection by M.grisea, while trehalose degradation is important for efficient development of the fungus in plant tissue following initial infection.
Links	PubMed PMC140093 Online version:10.1093/emboj/cdg018
Keywords	Amino Acid Sequence; Fungal Proteins/classification; Fungal Proteins/genetics; Fungal Proteins/metabolism; Gene Deletion; Genes, Fungal; Glucosyltransferases/genetics; Glucosyltransferases/metabolism; Magnaporthe/pathogenicity; Magnaporthe/physiology; Molecular Sequence Data; Open Reading Frames; Oryza sativa/microbiology; Phenotype; Phylogeny; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Sequence Alignment; Trehalase/chemistry; Trehalase/classification; Trehalase/genetics; Trehalase/metabolism; Trehalose/metabolism; Virulence
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MAGGR:Q875L7	involved_in	GO:0075039: establishment of turgor in appressorium	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
MAGGR:Q875L7	involved_in	GO:0006111: regulation of gluconeogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
MAGGR:Q875L7		GO:0006111: regulation of gluconeogenesis	ECO:0000315:		P		Table 1 shows tps1 KO mutants are unable to grow on glucose, sucrose, fructose, maltose, or trehalose. Additionally this strain could not grow on acetate or fatty acids.	complete CACAO 2511
MAGGR:Q875L7		GO:0075039: establishment of turgor in appressorium	ECO:0000315:		P		Figure 2c shows Appressorium turgor of tps1 KO mutants was significantly decreased compared to the WT.	complete CACAO 2513

References

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