PMID:12504017

Citation	Skaar, DA and Greenleaf, AL (2002) The RNA polymerase II CTD kinase CTDK-I affects pre-mRNA 3' cleavage/polyadenylation through the processing component Pti1p. Mol. Cell 10:1429-39
Abstract	There are several kinases in Saccharomyces cerevisiae that phosphorylate the CTD of RNA polymerase II, but specific and distinct functions of the phospho-CTDs generated by the different kinases are not well understood. A genetic screen for suppressors of loss of yeast CTD kinase I (CTDK-I) function (by deletion of the catalytic subunit gene CTK1) identified PTI1, a potential 3' cleavage/polyadenylation factor. Genetic and physical interactions connect Pti1p to components of CF IA and CF II/CPF, and mutations of PTI1 or CTK1 affect 3' cleavage site choice and transcript abundance of particular genes. Therefore, one important function of the CTDK-I-generated phospho-CTD appears to be the coupling of transcription to 3' processing of pre-mRNAs by a Pti1p-containing complex.
Links	PubMed
Keywords	Amino Acid Sequence; Genes, Essential; Genes, Fungal; Molecular Sequence Data; Peptide Fragments/chemistry; Poly A/metabolism; Protein Kinases/metabolism; Protein Subunits/chemistry; Protein Subunits/genetics; Protein Subunits/metabolism; Protein-Serine-Threonine Kinases/metabolism; RNA Polymerase II/chemistry; RNA Polymerase II/genetics; RNA Polymerase II/metabolism; RNA Precursors/metabolism; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae Proteins; Sequence Alignment; Sequence Homology, Amino Acid; Substrate Specificity; Transcription, Genetic; mRNA Cleavage and Polyadenylation Factors
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