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PMID:12439746

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Citation

Blondel, D, Regad, T, Poisson, N, Pavie, B, Harper, F, Pandolfi, PP, De Thé, H and Chelbi-Alix, MK (2002) Rabies virus P and small P products interact directly with PML and reorganize PML nuclear bodies. Oncogene 21:7957-70

Abstract

The interferon-induced promyelocytic leukaemia (PML) protein localizes both in the nucleoplasm and in matrix-associated multi-protein complexes known as nuclear bodies (NBs). NBs are disorganized in acute promyelocytic leukaemia or during some viral infections, suggesting that PML NBs could be a part of cellular defense mechanism. Rabies virus, a member of the rhabdoviridae family, replicates in the cytoplasm. Rabies phosphoprotein P and four other amino-terminally truncated products (P2, P3, P4, P5) are all translated from P mRNA. P and P2 are located in the cytoplasm, whereas P3, P4 and P5 are found mostly in the nucleus. Infection with rabies virus reorganized PML NBs. PML NBs became larger and appeared as dense aggregates when analysed by confocal or electron microscopy, respectively. The expression of P sequesters PML in the cytoplasm where both proteins colocalize, whereas that of P3 results in an increase in PML body size, as observed in infected cells. The P and P3 interacted directly in vivo and in vitro with PML. The C-terminal domain of P and the PML RING finger seem to be involved in this binding. Moreover, PML-/- primary mouse embryonic fibroblasts expressed viral proteins at a higher level and produced 20 times more virus than wild-type cells, suggesting that the absence of all PML isoforms resulted in an increase in rabies virus replication.

Links

PubMed Online version:10.1038/sj.onc.1205931

Keywords

Animals; Base Sequence; CHO Cells; Cricetinae; Cytoplasm/metabolism; DNA Primers; Neoplasm Proteins/metabolism; Nuclear Proteins; Phosphoproteins/metabolism; Protein Binding; Transcription Factors/metabolism; Tumor Suppressor Proteins; Viral Structural Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RABVC:PHOSP

GO:0075341: host cell PML body

ECO:0000314:

C

Figure 4a. The expression of P3 results in an increase in PML body size

complete
CACAO 8652

RABVC:PHOSP

Colocalizes with

GO:0075341: host cell PML body

ECO:0000314:

C

Figure 2. Rabies phosphoprotein colocalizes with host cell PMLs in the cytoplasm.

complete
CACAO 8651

RABVC:PHOSP

colocalizes_with

GO:0075341: host cell PML body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

See also

References

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