PMID:12430030

Citation	von Malek, B, van der Graaff, E, Schneitz, K and Keller, B (2002) The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway. Planta 216:187-92
Abstract	The Arabidopsis thaliana (L.) Heynh. mutant delayed-dehiscence2-2 (dde2-2) was identified in an En1/Spm1 transposon-induced mutant population screened for plants showing defects in fertility. The dde2-2 mutant allele is defective in the anther dehiscence process and filament elongation and thus exhibits a male-sterile phenotype. The dde2-2 phenotype can be rescued by application of methyl jasmonate, indicating that the mutant is affected in jasmonic acid biosynthesis. The combination of genetic mapping and a candidate-gene approach identified a frameshift mutation in the ALLENE OXIDE SYNTHASE (AOS) gene, encoding one of the key enzymes of jasmonic acid biosynthesis. Expression analysis and genetic complementation of the dde2-2 phenotype by overexpression of the AOS coding sequence confirmed that the male-sterile phenotype is indeed caused by the mutation in the AOS gene.
Links	PubMed Online version:10.1007/s00425-002-0906-2
Keywords	Acetic Acids/pharmacology; Amino Acid Sequence; Arabidopsis/drug effects; Arabidopsis/enzymology; Arabidopsis/genetics; Chromosome Mapping; Chromosomes, Plant/genetics; Cyclopentanes/metabolism; Cyclopentanes/pharmacology; Fertility/genetics; Genetic Complementation Test; Intramolecular Oxidoreductases/genetics; Intramolecular Oxidoreductases/metabolism; Molecular Sequence Data; Mutation; Oxylipins; Sequence Homology, Amino Acid
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