PMID:12427945

Citation	Morimoto, T, Loh, PC, Hirai, T, Asai, K, Kobayashi, K, Moriya, S and Ogasawara, N (2002) Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis. Microbiology (Reading, Engl.) 148:3539-52
Abstract	GTP-binding proteins are found in all domains of life and are involved in various essential cellular processes. With the recent explosion of available genome sequence data, a widely distributed bacterial subfamily of GTP-binding proteins was discovered, represented by the Escherichia coli Era and the Bacillus subtilis Obg proteins. Although only a limited number of the GTP-binding proteins belonging to the subfamily have been experimentally characterized, and their function remains unknown, the available data suggests that many of them are essential to bacterial growth. When the complete genomic sequence of B. subtilis was surveyed for genes encoding GTP-binding proteins of the Era/Obg family, nine such genes were identified. As a first step in elucidating the functional networks of those nine GTP-binding proteins, data presented here indicates that six of them are essential for B. subtilis viability. Additionally, it is shown that the six essential proteins are able to specifically bind GTP and GDP in vitro. Experimental depletion of the essential GTP-binding proteins was examined in the context of cell morphology and chromosome replication, and it was found that two proteins, Bex and YqeH, appeared to participate in the regulation of initiation of chromosome replication. Collectively, these results suggest that members of the GTP-binding Era/Obg family are important proteins with precise, yet still not fully understood, roles in bacterial growth and viability.
Links	PubMed
Keywords	Amino Acid Sequence; Bacillus subtilis/growth & development; Bacillus subtilis/physiology; Bacterial Proteins/chemistry; Bacterial Proteins/classification; Chromosomes, Bacterial/physiology; Escherichia coli Proteins; GTP-Binding Proteins/chemistry; GTP-Binding Proteins/classification; GTP-Binding Proteins/physiology; Guanosine Diphosphate/metabolism; Guanosine Triphosphate/metabolism; Molecular Sequence Data; Phylogeny; RNA-Binding Proteins; Sequence Homology, Amino Acid
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:ERA	involved_in	GO:0032297: negative regulation of DNA-dependent DNA replication initiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
BACSU:ERA		GO:0032297: negative regulation of DNA-dependent DNA replication initiation	ECO:0000314:		P		Figure 6B shows that the absence of Bex shifts the number of replication origins from 2-4 to 4+ in presence of IPTG. This is evidence that the absence of Bex increases initiation of chromosome replication.	complete CACAO 4228
BACSU:YQEH		GO:0032297: negative regulation of DNA-dependent DNA replication initiation	ECO:0000314:		P		Figure 6B shows that the absence of YqueH shifts the number of replication origins from 2-4 to 4+ in presence of IPTG. This is evidence that the absence of YqueH increases initiation of chromosome replication.	complete CACAO 4231
BACSU:YQEH	involved_in	GO:0032297: negative regulation of DNA-dependent DNA replication initiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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